منابع مشابه
Evaluation of post-thaw DNA integrity of mouse blastocysts after ultrarapid and slow freezing.
OBJECTIVE To evaluate the effect of vitrification and two other methods of slow cryopreservation on DNA integrity in expanded and nonexpanded blastocysts. DESIGN Prospective in vitro study. SETTING Tertiary care academic hospital. INTERVENTION(S) 1) Twenty-two expanded blastocysts (EB) and 17 nonexpanded blastocysts (NEB) vitrified in cryotips; 2) 15 EB and 16 NEB by slow freezing using p...
متن کاملDifferent chromatin and energy/redox responses of mouse morulae and blastocysts to slow freezing and vitrification
BACKGROUND The ability to cryopreserve mammalian embryos has become an integral part of assisted reproduction, both in human and veterinary medicine. Despite differences in the size and physiological characteristics of embryos from various species, the embryos have been frozen by either of two procedures: slow freezing or vitrification. The aim of our study was to compare the effect of slow fre...
متن کاملGene expression profiles of in vivo derived mouse blastocysts after slow-freezing.
The goals of our study were to analyze change of global gene expression profile of mouse blastocysts after slow-freezing and to explore molecular mechanism underlying the decreased pregnancy rate caused by cryopreservation. The results showed that superovulation differentially regulated the expression of 288 genes with at least 2.0-fold change considerations. Among which, 275 genes were down-re...
متن کاملSlow controlled-rate freezing of sequentially cultured human blastocysts: an evaluation of two freezing strategies.
BACKGROUND To optimize blastocyst cryopreservation, the prerequisite is to develop a better understanding of factors that influence their survival and implantation potential. Therefore, the aim of the present work was to evaluate, retrospectively, the outcome of blastocyst cryopreservation in a day 2/3 fresh embryo transfer programme. METHODS Two different freezing strategies were compared: a...
متن کاملP-67: Quantitative Expression of Pluripotency Specific-Genes in Mouse Blastocysts Produced by In Vitro Fertilization
Background: The efficiency of in vitro fertilization (IVF) is still low to be developed to blastocyst stage probably because of environmental conditions. It is likely that in vitro environment can not exactly mimic in vivo environment due to differences in media, metabolic content, atmospheric composition, temperature and pH. Therefore it may affect embryo quality by changing in embryo gene exp...
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ژورنال
عنوان ژورنال: Veterinary Quarterly
سال: 1982
ISSN: 0165-2176,1875-5941
DOI: 10.1080/01652176.1982.9693837